Chloroplast Subspecies-Specific SNP Detection and Its Maternal Inheritance in Brassica oleracea L. by Using a dCAPS Marker

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Chloroplast subspecies-specific SNP detection and its maternal inheritance in Brassica oleracea L. by using a dCAPS marker.

Chloroplast simple sequence repeats amplicons in 5 subspecies of Brassica oleracea were sequenced, and one chloroplast SNP was detected in amplicon ACP43. Through the introduction of an RsaI recognition site by adding one mismatch in the forward primer, combined with the increased primer length and raised annealing temperature, the dCAPS (derived cleaved amplified polymorphic sequences) marker ...

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ژورنال

عنوان ژورنال: Journal of Heredity

سال: 2012

ISSN: 1465-7333,0022-1503

DOI: 10.1093/jhered/ess006